Xie X, Clausen O P, De Angelis P, Boysen M
Department of Otolaryngology, The National Hospital, Rikshospitalet, University of Oslo, Norway.
Br J Cancer. 1998 Jul;78(1):100-5. doi: 10.1038/bjc.1998.449.
Using nucleolar organizer regions (NORs) as a proliferative marker and Bax expression as a marker for apoptosis, we have studied the individual and combined prognostic significance of these markers. Successive sections of diagnostic, formalin-fixed and paraffin-embedded specimens from 69 patients with T1-4 tumours were stained with a rabbit anti-human Bax polyclonal antibody and silver nitrate for visualization of NORs (AgNORs). After classification for staining intensity and the percentage of Bax expression, a final score resulting in four classes of increasing Bax expression was obtained. AgNOR counts were expressed as mean counts (mAgNOR) and the percentage of tumour nuclei with more than one AgNOR (pAgNOR>1). Both AgNOR parameters were grouped in three classes with increasing values. Low Bax scores correlated significantly with poor prognosis (P = 0.0106). For mAgNOR and pAgNOR>1, high values correlated with poor prognosis (P = 0.0185 and P = 0.0003 respectively). A combined parameter, for which the Bax score was subtracted from the AgNOR scores, appeared to be statistically stronger than the individual parameters (P < 0.0001). Both Bax expression and AgNOR scores, and in particular the combination of these parameters, appear to be strong prognostic markers in glottic squamous cell carcinomas.
我们以核仁组织区(NORs)作为增殖标志物,以Bax表达作为凋亡标志物,研究了这些标志物各自及联合应用时的预后意义。对69例患有T1 - 4期肿瘤患者的诊断性、经福尔马林固定及石蜡包埋标本的连续切片,用兔抗人Bax多克隆抗体进行染色,并用硝酸银对NORs(AgNORs)进行可视化处理。在对染色强度和Bax表达百分比进行分类后,得出了一个最终评分,该评分产生了四类Bax表达递增的情况。AgNOR计数表示为平均计数(mAgNOR)以及具有一个以上AgNOR的肿瘤细胞核百分比(pAgNOR>1)。这两个AgNOR参数均分为三类,数值递增。低Bax评分与预后不良显著相关(P = 0.0106)。对于mAgNOR和pAgNOR>1,高数值与预后不良相关(分别为P = 0.0185和P = 0.0003)。一个联合参数,即从AgNOR评分中减去Bax评分,在统计学上似乎比单个参数更强(P < 0.0001)。Bax表达和AgNOR评分,特别是这些参数的组合,似乎是声门鳞状细胞癌中强有力的预后标志物。