Kiel R A, Tanzer J M
Infect Immun. 1977 Sep;17(3):510-2. doi: 10.1128/iai.17.3.510-512.1977.
The regulation of diethylaminoethyl-partially purified invertase (EC 3.2.1.26; beta-D-fructofuranoside fructohydrolase) from the 37,000 X g-soluble intracellular fluid of Actinomyces viscosus serotype 2 strain M-100 was studied. Glycolytic intermediates, mono-, di-, and triphosphate nucleotides, inorganic phosphate, and various divalent cations were tested for regulatory effects. Fructose-6-phosphate (F6P) and fructose-1,6-diphosphate (FDP) were found to act as noncompetitive inhibitors of invertase. The Ki values for F6P and FDP were found to be 3.4 and 5.1 mM, respectively. The Hill coefficient for sucrose was 1.03 and remained unchanged in the presence of varying amounts of F6P or FDP.
对来自粘性放线菌血清型2菌株M-100的37,000×g可溶性细胞内液中的二乙氨基乙基部分纯化的转化酶（EC 3.2.1.26；β-D-呋喃果糖苷果糖水解酶）的调节作用进行了研究。测试了糖酵解中间产物、单磷酸、二磷酸和三磷酸核苷酸、无机磷酸盐以及各种二价阳离子的调节作用。发现6-磷酸果糖（F6P）和1,6-二磷酸果糖（FDP）作为转化酶的非竞争性抑制剂。F6P和FDP的Ki值分别为3.4和5.1 mM。蔗糖的希尔系数为1.03，在存在不同量的F6P或FDP时保持不变。
