van Diest P J, Michalides R J, Jannink L, van der Valk P, Peterse H L, de Jong J S, Meijer C J, Baak J P
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Am J Pathol. 1997 Feb;150(2):705-11.
Cyclin D1 overexpression, detected by standard immunohistochemistry, was correlated with other prognostic variables and its prognostic value was evaluated in a group of 148 invasive breast cancers with long-term follow-up. Overexpression of cyclin D1 (59% of cases) was negatively correlated (chi 2 test) with histological grade (P = 0.0001), mean nuclear area (P = 0.004), mean nuclear volume (P = 0.02), and mitotic activity (P = 0.03) and positively correlated with estrogen receptor (P = 0.0001). There was a strong correlation between cyclin D1 overexpression and histological type (P = 0.0001). Positive cyclin D1 staining was seen in 11 of 13 tubular carcinomas, 3 of 3 mucinous carcinomas, 4 of 4 invasive cribriform carcinomas, and 17 of 20 lobular carcinomas. Of 102 ductal cancers, 52 were positive, and all 6 medullary carcinomas were negative. There were no significant correlations with lymph node status, tumor size, or DNA ploidy. In survival analysis, cyclin D1 overexpression did not provide significant univariate or multivariate prognostic value. In conclusion, cyclin D1 is mainly overexpressed in the well differentiated and lobular types of invasive breast cancer and is strongly associated with estrogen receptor positivity. It is negatively correlated with the proliferation marker mitoses count and with the differentiation markers nuclear area and nuclear volume. However, cyclin D1 overexpression does not seem to have prognostic value in invasive breast cancer when no adjuvant treatment is given.
通过标准免疫组织化学检测发现，细胞周期蛋白D1过表达与其他预后变量相关，并在一组148例接受长期随访的浸润性乳腺癌中评估了其预后价值。细胞周期蛋白D1过表达（59%的病例）与组织学分级（χ²检验，P = 0.0001）、平均核面积（P = 0.004）、平均核体积（P = 0.02）和有丝分裂活性（P = 0.03）呈负相关，与雌激素受体呈正相关（P = 0.0001）。细胞周期蛋白D1过表达与组织学类型之间存在强相关性（P = 0.0001）。在13例管状癌中有11例、3例黏液癌中有3例、4例浸润性筛状癌中有4例以及20例小叶癌中有17例可见细胞周期蛋白D1阳性染色。在102例导管癌中，有52例呈阳性，所有6例髓样癌均为阴性。与淋巴结状态、肿瘤大小或DNA倍体无显著相关性。在生存分析中，细胞周期蛋白D1过表达未提供显著的单变量或多变量预后价值。总之，细胞周期蛋白D1主要在分化良好的浸润性乳腺癌小叶类型中过表达，且与雌激素受体阳性密切相关。它与增殖标志物有丝分裂计数以及分化标志物核面积和核体积呈负相关。然而，在未进行辅助治疗的浸润性乳腺癌中，细胞周期蛋白D1过表达似乎没有预后价值。
