Misaki A, Goldstein I J
J Biol Chem. 1977 Oct 25;252(20):6995-9.
Pronase digestion of the lima bean lectin (Phaseolus lunatus (alpha-D-GalNAc greater than alpha-D-Gal)) afforded a glycopeptide of molecular weight 1380. Amino acid and carbohydrate analysis showed the glycopeptide to contain fucose, mannose, glucosamine, and aspartic acid in the molar ratios, 0.8:4.4:1.9:1.0. Methylation analysis, Smith degradation, and sequential degradation by specific glycosidases permit the formulation of a tentative structure:.
用链霉蛋白酶消化利马豆凝集素(菜豆(α-D-氨基半乳糖>α-D-半乳糖))得到一种分子量为1380的糖肽。氨基酸和碳水化合物分析表明,该糖肽含有岩藻糖、甘露糖、葡糖胺和天冬氨酸,摩尔比为0.8:4.4:1.9:1.0。甲基化分析、史密斯降解以及特定糖苷酶的顺序降解有助于构建一个初步结构: