Fontan E, Briend E, Saklani-Jusforgues H, Fauve R M
Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.
Int J Cancer. 1997 Feb 7;70(4):416-22. doi: 10.1002/(sici)1097-0215(19970207)70:4<416::aid-ijc8>3.0.co;2-z.
The ability of a purified human glycoprotein (HGP.92) to exert anti-tumor activity was investigated in a mouse model using long-term readout assays. In vitro, in the presence of inflammatory mouse macrophages incubated with HGP.92, the growth of the mouse Lewis-lung-tumor cells (3LL) was decreased. This effect was concentration-dependent and required direct contact between tumor targets and HGP.92-treated macrophages. In addition, if the macrophage monolayer was depleted of HGP.92 before addition of the target cells, no more cytostatic effect was observed. This anti-tumor activity of HGP.92-treated mouse macrophage was partially abrogated by addition of catalase in the culture medium, but not by superoxide dismutase or scavengers of the hydroxyl radical and singlet oxygen. Moreover, this tumor-cell growth reduction was not dependent on nitric oxide. In vivo, multiple i.v. injections of HGP.92 (5 times, 3 days apart) during the first week and a half exerted significant anti-tumor activity, as assessed by the reduction of both the number and the size of the lung nodules 3 weeks after i.v. inoculation of 3LL cells.
使用长期读数测定法在小鼠模型中研究了纯化的人糖蛋白（HGP.92）发挥抗肿瘤活性的能力。在体外，在与HGP.92孵育的炎性小鼠巨噬细胞存在下，小鼠Lewis肺癌细胞（3LL）的生长减少。这种作用是浓度依赖性的，并且需要肿瘤靶标与HGP.92处理的巨噬细胞之间直接接触。此外，如果在添加靶细胞之前巨噬细胞单层中耗尽了HGP.92，则不再观察到细胞生长抑制作用。添加过氧化氢酶可部分消除HGP.92处理的小鼠巨噬细胞的这种抗肿瘤活性，但超氧化物歧化酶或羟基自由基和单线态氧的清除剂则不能。此外，这种肿瘤细胞生长的减少不依赖于一氧化氮。在体内，在第一周半期间多次静脉注射HGP.92（5次，间隔3天）发挥了显著的抗肿瘤活性，这通过静脉注射3LL细胞3周后肺结节的数量和大小的减少来评估。
