Fontan E, Briend E, Saklani-Jusforgues H, d'Alayer J, Vandekerckhove J, Fauve R M
Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.
Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8353-7. doi: 10.1073/pnas.91.18.8353.
We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 micrograms/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety.
我们将一种92kDa的人尿糖蛋白（HGP.92）纯化至表观均一，以250微克/千克的剂量静脉注射该蛋白时，能使小鼠完全抵御致死剂量的单核细胞增生李斯特菌接种。由于HGP.92能保护缺乏B淋巴细胞和T淋巴细胞的重症联合免疫缺陷（scid）小鼠，这种对李斯特菌抵抗力的增强似乎并非由淋巴细胞介导。此外，用6 nM HGP.92孵育的炎性巨噬细胞在体外可抑制刘易斯癌细胞的生长。这两种活性似乎都依赖于一个寡糖部分，因为在对HGP.92进行N - 聚糖酶处理后它们丧失了。因此，HGP.92的生物活性在某种程度上与一个聚糖部分有关。
