从酵母到人类的线粒体DNA聚合酶：一个新的聚合酶家族。

Mitochondrial DNA polymerases from yeast to man: a new family of polymerases.

作者信息

Lecrenier N, Van Der Bruggen P, Foury F

机构信息

Laboratoire de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.

出版信息

Gene. 1997 Jan 31;185(1):147-52. doi: 10.1016/s0378-1119(96)00663-4.

DOI:10.1016/s0378-1119(96)00663-4

PMID:9034326

Abstract

We report the sequence of a 4.5-kb cDNA clone isolated from a human melanoma library which bears high amino acid sequence identity to the yeast mitochondrial (mt) DNA polymerase (Mip1p). This cDNA contains a 3720-bp open reading frame encoding a predicted 140-kDa polypeptide that is 43% identical to Mip1p. The N-terminal part of the sequence contains a 13 glutamine stretch encoded by a CAG trinucleotide repeat which is not found in the other DNA polymerases gamma (Pol gamma). Multiple amino acid sequence alignments with Pol gamma from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, Drosophila melanogaster, Xenopus laevis and Mus musculus show that these DNA polymerases form a family strongly conserved from yeast to man and are only loosely related to the Family A DNA polymerases.

摘要

我们报道了从人黑色素瘤文库中分离出的一个4.5kb cDNA克隆的序列，该克隆与酵母线粒体（mt）DNA聚合酶（Mip1p）具有高度的氨基酸序列同一性。这个cDNA包含一个3720bp的开放阅读框，编码一个预测的140kDa多肽，该多肽与Mip1p有43%的同一性。序列的N端部分包含由CAG三核苷酸重复序列编码的13个谷氨酰胺延伸，这在其他DNA聚合酶γ（Polγ）中未发现。与酿酒酵母、粟酒裂殖酵母、巴斯德毕赤酵母、黑腹果蝇、非洲爪蟾和小家鼠的Polγ进行的多氨基酸序列比对表明，这些DNA聚合酶形成了一个从酵母到人类都高度保守的家族，并且与A家族DNA聚合酶的关系较为松散。