Villena A, Zapata A, Rivera-Pomar J M, Barrutia M G, Fonfria J
Cell Tissue Res. 1983;229(1):219-32. doi: 10.1007/BF00217894.
This study describes the postnatal development of the nonlymphoid cells with special reference to the fibroblastic reticulum cells (FRCs) and interdigitating cells (IDCs). The first lymphocytes of the neonatal lymph nodes are located in the developing deep cortex units (DCUs) identified by the Gomori's technique for reticulin fibres. Ultrastructural studies demonstrate that FRCs form the stroma of the DCUs. By light and electron microscopy, it is demonstrated that FRCs occupy the outer cortex in the following stages of development of the lymph nodes. Thus, FRCs form the stroma of the primary follicles and, later, are transformed in follicular dendritic cells (FDCs) of the germinal centres. Immature or pro-IDCs appear as migrating elements in the deep cortex of lymph nodes of the neonatal rats. The ultrastructure of the pro-IDCs resembles that of the mature IDCs but not that of the phagocytic cells. Pro-IDCs are transformed into mature IDCs whose cytoplasmic expansions contact lymphocytes via tight junctions. Some of these lymphocytes are likely apposed to FRCs of the DCUs. No cells containing Birbeck granules were found in the parenchyma of the lymph nodes during the postnatal development. The role of these nonlymphoid cells is discussed with respect to the immunologic function of mammalian lymph nodes.
本研究描述了非淋巴细胞的出生后发育，特别提及了成纤维网状细胞（FRCs）和交错突细胞（IDCs）。新生淋巴结的首批淋巴细胞位于通过戈莫里网状纤维技术鉴定的发育中的深层皮质单位（DCUs）中。超微结构研究表明，FRCs构成了DCUs的基质。通过光镜和电镜观察发现，在淋巴结发育的以下阶段，FRCs占据外皮质。因此，FRCs形成初级滤泡的基质，随后转变为生发中心的滤泡树突状细胞（FDCs）。未成熟或前IDCs在新生大鼠淋巴结深层皮质中表现为迁移成分。前IDCs的超微结构类似于成熟IDCs，但不同于吞噬细胞。前IDCs转变为成熟IDCs，其细胞质突起通过紧密连接与淋巴细胞接触。其中一些淋巴细胞可能与DCUs的FRCs相邻。在出生后发育过程中，淋巴结实质内未发现含有伯贝克颗粒的细胞。就哺乳动物淋巴结的免疫功能而言，讨论了这些非淋巴细胞的作用。
