具有酵母杀伤毒素活性的抗独特型单链抗体的治疗潜力。
Therapeutic potential of antiidiotypic single chain antibodies with yeast killer toxin activity.
作者信息
Magliani W, Conti S, de Bernardis F, Gerloni M, Bertolotti D, Mozzoni P, Cassone A, Polonelli L
机构信息
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Parma, Italy.
出版信息
Nat Biotechnol. 1997 Feb;15(2):155-8. doi: 10.1038/nbt0297-155.
Single chain fragment (ScFv) antiidiotypic antibodies (antilds) of a killer toxin (KT) from the yeast Pichia anomala have been produced by recombinant DNA methodology from the splenic lymphocytes of mice immunized by idiotypic vaccination with a KT-neutralizing monoclonal antibody (Mab KT4). ScFv KT-like antilds (KTIdAb) react with specific Candida albicans KT cell wall receptors (KTR) exerting a candidacidal activity in vitro could be neutralized by adsorption with Mab KT4. ScFv KTIdAb displayed an effective therapeutic activity in an experimental model of rat candidal vaginitis.
通过重组DNA方法，利用用抗独特型疫苗接种的KT中和单克隆抗体（Mab KT4）免疫的小鼠脾淋巴细胞，制备了来自异常毕赤酵母杀伤毒素（KT）的单链片段（ScFv）抗独特型抗体（抗Id）。ScFv KT样抗Id（KTIdAb）与白色念珠菌KT细胞壁受体（KTR）特异性反应，在体外发挥杀念珠菌活性，可被Mab KT4吸附中和。ScFv KTIdAb在大鼠念珠菌性阴道炎实验模型中显示出有效的治疗活性。
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