Bartolozzi F, Bertazza G, Bassi D, Cristoferi G
Departimento di Colture Arboree, Università di Bologna, Italy.
J Chromatogr A. 1997 Jan 10;758(1):99-107. doi: 10.1016/s0021-9673(96)00709-1.
The aim of this study was to determine a reliable procedure for the quantification of organic acids, alcohol soluble sugars and sugar alcohols in fruit flesh by means of a rapid GLC method, without resorting to methoximation of sugars and employing apricot as a model. The use of two internal standards, an accurate derivatization and a proper calibration of the GLC conditions allowed an accurate quantitative analysis of the compounds detected in the unknown samples. This simple procedure improves the speed of preparation of the trimethylsilyl derivatives and is highly reproducible. Variability was found between years for each of the five cultivars studied and for each compound in terms of absolute values, whereas the percentage incidence of the single sugars as a total was more stable over the two years of observation.
本研究的目的是确定一种可靠的程序，通过快速气相色谱法对果肉中的有机酸、醇溶性糖和糖醇进行定量分析，无需对糖进行甲氧基化处理，并以杏作为模型。使用两种内标、准确的衍生化方法以及对气相色谱条件进行适当校准，能够对未知样品中检测到的化合物进行准确的定量分析。这种简单的程序提高了三甲基硅烷基衍生物的制备速度，并且具有高度的可重复性。在所研究的五个品种中的每一个品种以及每一种化合物方面，发现不同年份之间在绝对值上存在差异，而在两年的观察期内，单糖占总量的百分比发生率则更为稳定。
