Füzfai Zsófia, Katona Zsolt F, Kovács Etelka, Molnár-Perl Ibolya
Institute of Inorganic and Analytical Chemistry, L. Eötvös University, H-1518, Budapest 112, POB 32, Hungary.
J Agric Food Chem. 2004 Dec 15;52(25):7444-52. doi: 10.1021/jf040118p.
Our gas chromatography-mass spectrometry method--developed for the simultaneous quantitation of mono-, di-, and trisaccharides, sugar alcohols, caboxylic and amino acids, measured as their trimethylsilyl-(oxime) ether/ester derivatives, from one solution by a single injection, prepared in the presence of the fruit matrix--has been extended/utilized for special purposes. The compositions of (i) freshly harvested and stored sour cherries (Prunus cerasus), (ii) apples obtained from organic and integrated productions (Malus domestica), and (iii) green and ripe bers (Zizyphus mauritiana L.) were compared. On the basis of earlier, basic researches (derivatization, quantitation, and fragmentation studies of authentic compounds), we demonstrate the reproducible quantitation of the main and minor constituents in a wide concentration range (approximately 1 x 10(-)(3) to >/=40%, in total up to < or =98%, calculated on dry matter basis of the fruit matrices). Reproducibility of quantitations, calculated on the basis of their total ion current values, provided an average reproducibility of 3.3 (sour cherries), 6.2 (apple), and 4.3 (ber) RSD %, respectively.
我们开发的气相色谱-质谱法可通过一次进样从一份在水果基质存在下制备的溶液中同时定量测定单糖、二糖和三糖、糖醇、羧酸和氨基酸，这些物质以其三甲硅烷基-（肟）醚/酯衍生物的形式进行测定。该方法已被扩展/用于特殊目的。比较了（i）新鲜采摘和储存的酸樱桃（欧洲酸樱桃）、（ii）有机生产和综合生产的苹果（苹果属）以及（iii）青枣和成熟枣（毛叶枣）的成分。基于早期的基础研究（真实化合物的衍生化、定量和碎片研究），我们证明了在宽浓度范围内（按水果基质干物质计算，约为1×10⁻³至≥40%，总计高达≤98%）主要和次要成分的可重复定量。基于总离子流值计算的定量重现性分别提供了3.3（酸樱桃）、6.2（苹果）和4.3（枣）%的平均相对标准偏差。
