Prins M, Kikkert M, Ismayadi C, de Graauw W, de Haan P, Goldbach R
Department of Virology, Wageningen Agricultural University, Netherlands.
Plant Mol Biol. 1997 Jan;33(2):235-43. doi: 10.1023/a:1005729808191.
Transgenic Nicotiana tabacum plants expressing RNA sequences of the tomato spotted wilt virus NS(M) gene, which encodes the putative viral movement protein, were found to be highly resistant to infection with the virus. Expression of untranslatable as well as anti-sense RNA of the NS(M) gene resulted in resistance levels as high as those in plants expressing translatable RNA sequences. For all three types of transgenic plants resistance levels of up to 100% were reached in the S2 progeny. These results indicate that the resistance mediated by the NS(M) gene is accomplished by expression of transcripts rather than protein in transgenic plants, similar to previously observed N gene-mediated resistance. Protoplast inoculations revealed that resistant plants expressing NS(M) are, in contrast to N transgenic resistant plants, not resistant at the cellular level. This suggests the RNA-mediated resistance mechanism against TSWV targets viral mRNAs rather than the viral genome.
表达番茄斑萎病毒NS(M)基因RNA序列（该基因编码假定的病毒运动蛋白）的转基因烟草植株被发现对该病毒感染具有高度抗性。NS(M)基因不可翻译的RNA以及反义RNA的表达所产生的抗性水平与表达可翻译RNA序列的植株一样高。对于所有三种类型的转基因植株，在S2子代中抗性水平高达100%。这些结果表明，NS(M)基因介导的抗性是通过转基因植株中转录本而非蛋白质的表达来实现的，这与之前观察到的N基因介导的抗性类似。原生质体接种显示，与N转基因抗性植株不同，表达NS(M)的抗性植株在细胞水平上并不具有抗性。这表明针对番茄斑萎病毒的RNA介导抗性机制作用于病毒mRNA而非病毒基因组。
