Schlicher T, Soll J
Botanisches Institut, Universitat Kiel, Germany.
Plant Mol Biol. 1997 Jan;33(1):181-5. doi: 10.1023/a:1005784115363.
The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ homologue and present evidence that the gene product is at least partially associated with the inner envelope membrane. Immunoblot analysis also provides evidence for the presence of a GrpE homologue in plastids.
细胞中蛋白质的折叠和组装通常需要分子伴侣的协助，如Hsp70和Hsp60热休克蛋白。Hsp70伴侣蛋白与DnaJ和GrpE同源物协同作用，以确保高效的折叠循环。在本研究中，我们描述了首个定位于叶绿体的DnaJ同源物的基因，并提供证据表明该基因产物至少部分与内膜相关。免疫印迹分析也为质体中存在GrpE同源物提供了证据。
