Bessoule J J
Plant Research Laboratory, Michigan State University.
FEBS Lett. 1993 May 24;323(1-2):51-4. doi: 10.1016/0014-5793(93)81446-7.
Antibodies raised against a purified fraction from microsomal membranes of leek epidermal cells were used to screen a lambda zap expression library from epidermal cells of leak plants. A near full-length clone was isolated. This cDNA contains an open reading-frame of 1,191 bp coding for a DnaJ protein (leek DNAJ 1 or LDJ1). Leek DnaJ1 represents the second protein of this type described in a pluricellular organism, the first being that sequenced from human cells.
用针对韭菜表皮细胞微粒体膜纯化组分产生的抗体，筛选来自韭菜植株表皮细胞的λ噬菌体Zap表达文库。分离出一个近乎全长的克隆。该cDNA包含一个1191bp的开放阅读框，编码一种DnaJ蛋白（韭菜DnaJ1或LDJ1）。韭菜DnaJ1是多细胞生物中描述的第二种这类蛋白，第一种是从人类细胞中测序得到的。
