脂质体介导的DNA疫苗接种。

Liposome-mediated DNA vaccination.

作者信息

Gregoriadis G, Saffie R, de Souza J B

机构信息

The School of Pharmacy, University of London, UK.

出版信息

FEBS Lett. 1997 Feb 3;402(2-3):107-10. doi: 10.1016/s0014-5793(96)01507-4.

DOI:10.1016/s0014-5793(96)01507-4

PMID:9037176

Abstract

Numerous reports have indicated that intramuscular injection of antigen-coding naked plasmid DNA can trigger humoral and cell-mediated protective immunity against infection. This follows DNA uptake by muscle fibres, leading to the expression and extracellular release of the antigen. Here it is shown for the first time that intramuscular immunization of mice with pRc/CMV HBS (encoding the S region of hepatitis B antigen; HBsAg) entrapped into positively charged (cationic) liposomes leads to greatly improved humoral and cell-mediated immunity. These cationic liposome-entrapped DNA vaccines generate titres of anti-HBsAg IgG1 antibody isotype in excess of 100-fold higher and increased levels of both IFN-gamma and IL-4 when compared with naked DNA or DNA complexed with preformed similar (cationic) liposomes. It is likely that immunization with liposome-entrapped plasmid DNA involves antigen-presenting cells locally or in the regional draining lymph nodes.

摘要

众多报告表明，肌肉注射编码抗原的裸质粒DNA可引发针对感染的体液免疫和细胞介导的保护性免疫。这是由于肌肉纤维摄取DNA，导致抗原的表达和细胞外释放。本文首次表明，将包裹于带正电荷（阳离子）脂质体中的pRc/CMV HBS（编码乙肝抗原的S区；HBsAg）对小鼠进行肌肉免疫，可显著提高体液免疫和细胞介导的免疫。与裸DNA或与预先形成的类似（阳离子）脂质体复合的DNA相比，这些包裹于阳离子脂质体中的DNA疫苗产生的抗HBsAg IgG1抗体亚型滴度高出100倍以上，且IFN-γ和IL-4水平均有所升高。用包裹于脂质体的质粒DNA进行免疫很可能涉及局部或区域引流淋巴结中的抗原呈递细胞。