Gast R K
USDA, Southeast Poultry Research Laboratory, Athens, Georgia 30605, USA.
Poult Sci. 1997 Jan;76(1):17-23. doi: 10.1093/ps/76.1.17.
Despite extensive testing for Salmonella pullorum infections in commercial poultry, occasional outbreaks of pullorum disease still occur. In some recent instances, questions have been raised about whether standard serological assays, which employ antigen strains selected several decades ago, are still able to detect currently prevalent S. pullorum strains. The present study evaluated the ability of standard rapid whole-blood plate agglutination test and serum tube agglutination test antigens to detect infection in Single Comb White Leghorn hens inoculated with large oral doses of six recent S. pullorum isolates. Two commercially available plate test antigens were obtained and three tube test antigens were prepared. All five antigens identified most inoculated hens as seropositive, although some differences in sensitivity were evident between the two assay types and between the two plate test antigens. The antigenic composition of the tube test antigens did not affect their ability to detect infections with the various inoculum strains. Regardless of the antigen used, hens infected with antigenically intermediate or variant S. pullorum strains were detected as seropositive less often than were hens infected with antigenically standard strains. Positive culturing results for S. pullorum in the livers and ovaries of infected hens were nearly always predicted by positive serological test results.
尽管对商业家禽中的鸡白痢感染进行了广泛检测，但鸡白痢疾病仍偶尔爆发。在最近的一些案例中，有人质疑使用几十年前选定的抗原菌株的标准血清学检测方法是否仍能检测出当前流行的鸡白痢菌株。本研究评估了标准快速全血平板凝集试验和血清试管凝集试验抗原对经大剂量口服接种六种近期鸡白痢分离株的单冠白来航母鸡感染情况的检测能力。获得了两种市售平板试验抗原，并制备了三种试管试验抗原。所有五种抗原都将大多数接种母鸡鉴定为血清阳性，尽管两种检测类型之间以及两种平板试验抗原之间在敏感性上存在一些明显差异。试管试验抗原的抗原组成并不影响它们检测各种接种菌株感染的能力。无论使用何种抗原，感染抗原性中间型或变异型鸡白痢菌株的母鸡被检测为血清阳性的频率都低于感染抗原性标准菌株的母鸡。感染母鸡肝脏和卵巢中鸡白痢的阳性培养结果几乎总是可以通过血清学检测阳性结果预测出来。
