Tsai C R, Garcia J L, Patel B K, Cayol J L, Baresi L, Mah R A
Department of Environmental Health Sciences, School of Public Health, University of California, Los Angeles 90024-1772, USA.
Int J Syst Bacteriol. 1995 Apr;45(2):301-7. doi: 10.1099/00207713-45-2-301.
A strictly anaerobic, moderately halophilic, gram-negative, rod-shaped bacterium was isolated from Great Salt Lake, Utah, sediments and designated GSLST (T = type strain). Strain GSLST grew optimally at pH 6.7 to 7.0 but had a very broad pH range for growth (pH 5.8 to 10.0). The optimum temperature for growth was 37 degrees C, and no growth occurred at 15 or 55 degrees C. The optimum salt concentration for growth was 10%. Strain GSLST required yeast extract and Trypticase peptone to ferment carbohydrates, pyruvate, and glycine betaine. Strain GSLST was resistant to penicillin, D-cycloserine, tetracycline, and streptomycin. The G + C content of this isolate was 31 mol%. The fermentation products from glucose utilization were acetate, butyrate, lactate, CO2, and H2, and in addition strain GSLST fermented glycine betaine to acetate and trimethylamine. All of these traits distinguish this organism from all previously described halophilic anaerobes. The 16S rRNA gene sequence of strain GSLST was found to be similar to, but also significantly different from, the 16S rRNA sequences of Haloanaerobium salsugo and Haloanaerobium praevalens. Therefore, strain GSLST (= DSM 8275T) is described as a new species, Haloanaerobium alcaliphilum.
从美国犹他州大盐湖沉积物中分离出一种严格厌氧、中度嗜盐、革兰氏阴性、杆状细菌，并将其命名为GSLST（T = 模式菌株）。菌株GSLST在pH 6.7至7.0条件下生长最佳，但生长的pH范围非常宽（pH 5.8至10.0）。生长的最适温度为37℃，在15℃或55℃下不生长。生长的最适盐浓度为10%。菌株GSLST需要酵母提取物和胰蛋白酶蛋白胨来发酵碳水化合物、丙酮酸和甘氨酸甜菜碱。菌株GSLST对青霉素、D-环丝氨酸、四环素和链霉素具有抗性。该分离株的G + C含量为31 mol%。利用葡萄糖产生的发酵产物为乙酸盐、丁酸盐、乳酸盐、CO2和H2，此外，菌株GSLST还将甘氨酸甜菜碱发酵为乙酸盐和三甲胺。所有这些特征将该生物体与所有先前描述的嗜盐厌氧菌区分开来。发现菌株GSLST的16S rRNA基因序列与盐沼嗜盐厌氧菌和前嗜盐厌氧菌的16S rRNA序列相似，但也有显著差异。因此，菌株GSLST（= DSM 8275T）被描述为一个新物种，嗜碱嗜盐厌氧菌。
