Cayol J L, Ollivier B, Patel B K, Ageron E, Grimont P A, Prensier G, Garcia J L
Laboratoire de Microbiologie ORSTOM, Université de Provence, Marseille, France.
Int J Syst Bacteriol. 1995 Oct;45(4):790-7. doi: 10.1099/00207713-45-4-790.
A new extremely halophilic chemoorganotrophic bacterium (strain H200T [T = type strain]) was isolated from the hypersaline sediments of Retba Lake in Senegal. This organism was a sluggishly motile, rod-shaped, non-spore-forming, gram-negative, obligate anaerobe that grew optimally at 40 degrees C in the presence of 180 to 200 g of NaCl per liter. The DNA base composition was 32 mol% guanine plus cytosine. The fermentation products from glucose were ethanol, acetate, H2, and CO2. Yeast extract was required for growth. The fermentable substrates included D-fructose, galactose, D-xylose, cellobiose, lactose, maltose, sucrose, starch, D-mannitol, glycerol, and Casamino Acids. On the basis of the results of a 16S rRNA sequence analysis, strain H200T was found to be related to Haloanaerobium species. The 16S rRNA sequence of strain H200T differed from the sequences of the three previously described Haloanaerobium species, and strain H200T also differed from these organisms in its NaCl range for growth (60 to 340 g/liter); strain H200T grew in the presence of the highest NaCl concentration recorded for any halophilic anaerobic organism, including the three previously described Haloanaerobium species. We propose that strain H200T (= DSM 10165) belongs to a new Haloanaerobium species, Haloanaerobium lacusroseus.
从塞内加尔雷特巴湖的高盐沉积物中分离出一种新型的嗜盐化能有机营养细菌（菌株H200T [T = 模式菌株]）。该菌运动迟缓，呈杆状，不形成芽孢，革兰氏阴性，专性厌氧菌，在每升含有180至200克氯化钠的条件下，40℃生长最佳。DNA碱基组成为32摩尔%的鸟嘌呤加胞嘧啶。葡萄糖发酵产物为乙醇、乙酸、氢气和二氧化碳。生长需要酵母提取物。可发酵底物包括D-果糖、半乳糖、D-木糖、纤维二糖、乳糖、麦芽糖、蔗糖、淀粉、D-甘露醇、甘油和酪蛋白氨基酸。基于16S rRNA序列分析结果，发现菌株H200T与嗜盐厌氧菌属的物种相关。菌株H200T的16S rRNA序列与先前描述的三种嗜盐厌氧菌属物种的序列不同，并且菌株H200T在其生长的氯化钠浓度范围（60至34克/升）上也与这些生物不同；菌株H200T能在任何嗜盐厌氧生物（包括先前描述的三种嗜盐厌氧菌属物种）所记录的最高氯化钠浓度下生长。我们提议菌株H200T（= DSM 10165）属于一个新的嗜盐厌氧菌属物种，命名为玫瑰湖嗜盐厌氧菌。
