Sammut B, Laurens V, Tournefier A
Laboratoire d'Immunologie Comparée, CNRS-UMR 5548, Université de Bourgogne, 6 Boulevard Gabriel, 21000 Dijon, France.
Immunogenetics. 1997;45(5):285-94. doi: 10.1007/s002510050207.
Class I major histocompatibility complex (Mhc) cDNA clones were isolated from axolotl mRNA by polymerase chain reaction (PCR) and by screening a cDNA phage library. The nucleotide and predicted amino acid sequences show definite similarities to the Mhc class Ialpha molecules of higher vertebrates. Most of the amino acids in the peptide binding region that dock peptides at their N and C termini in mammals are conserved. Several amino acids considered to be important for the interaction of beta2-microglobulin with the Mhc alpha chain are also conserved in the axolotl sequence. The fact that axolotl class I A cDNAs are ubiquitously expressed and highly polymorphic in the alpha1 and alpha2 domains suggests the classical nature of axolotl class I A genes.
通过聚合酶链反应（PCR）并筛选cDNA噬菌体文库，从蝾螈mRNA中分离出I类主要组织相容性复合体（Mhc）cDNA克隆。核苷酸和预测的氨基酸序列与高等脊椎动物的Mhc I类α分子显示出明确的相似性。在哺乳动物中，肽结合区域中在其N和C末端对接肽的大多数氨基酸是保守的。在蝾螈序列中，一些被认为对β2-微球蛋白与Mhcα链相互作用很重要的氨基酸也保守。蝾螈I类A cDNA在α1和α2结构域中普遍表达且高度多态，这一事实表明蝾螈I类A基因具有经典性质。
