Sigounas G, Steiner M, Anagnostou A
Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina, USA.
Angiology. 1997 Feb;48(2):141-7. doi: 10.1177/000331979704800207.
Endothelial cells, which are nonhemopoietic cells, express and/or produce most of the known hemopoietic receptors and cytokines. The biological role of these factors, and their respective receptors, on endothelial cells is still unknown. In this study, the authors assessed the effect of different hemopoietic growth factors, ie, interleukin-3 (IL-3), erythropoietin (EPO), macrophage-colony stimulating factor (M-CSF), granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), singly or in conjunction with others, on proliferation and chemotaxis of human umbilical vein endothelial cells (HUVECs). They found growth stimulatory activity with IL-3, EPO, and GM-CSF and potent synergism between EPO and IL-3, less with IL-3 and GM-CSF, and none with EPO and either GM-CSF or G-CSF. All the singly tested hemopoietic growth factors stimulated the migration of HUVECs, but in conjunction with other factors, they did not show any additive or synergistic effect.
内皮细胞属于非造血细胞，可表达和/或产生大多数已知的造血受体和细胞因子。这些因子及其各自的受体在内皮细胞上的生物学作用仍不清楚。在本研究中，作者评估了不同造血生长因子，即白细胞介素-3（IL-3）、促红细胞生成素（EPO）、巨噬细胞集落刺激因子（M-CSF）、粒细胞集落刺激因子（G-CSF）、粒细胞巨噬细胞集落刺激因子（GM-CSF）单独或联合作用对人脐静脉内皮细胞（HUVECs）增殖和趋化性的影响。他们发现IL-3、EPO和GM-CSF具有生长刺激活性，EPO和IL-3之间有显著协同作用，IL-3和GM-CSF之间协同作用较弱，EPO与GM-CSF或G-CSF之间无协同作用。所有单独测试的造血生长因子均能刺激HUVECs迁移，但与其他因子联合时，未显示出任何相加或协同效应。
