Rittig M G, Kuhn K H, Dechant C A, Gauckler A, Modolell M, Ricciardi-Castagnoli P, Krause A, Burmester G R
Department of Anatomy I, University of Erlangen, Germany.
Dev Comp Immunol. 1996 Nov-Dec;20(6):393-406. doi: 10.1016/s0145-305x(96)00023-7.
Coiling phagocytosis has been observed previously only by chance, and there has been no systematic investigation of this uptake mechanism. Therefore, a comparative electron microscopical study was performed. Different human and murine cell populations, phagocytes from various vertebrate and invertebrate species, and predatory amoebae were incubated with Borrelia burgdorferi, one of the microbes known to induce coiling phagocytosis, to study the uptake mechanisms used. In this model, coiling phagocytosis was observed with both vertebrate and invertebrate species but not with amoebae. With cells from humans and mice, this uptake mechanism was restricted to phagocytic cells of myeloid origin. The coiled membrane gaps did not give rise to phagosomes; instead, membrane fusion was followed by membrane dissipation. Thus, coiling of B. burgdorferi apparently is an alternative uptake mechanism used by metazoan phagocytes, involving special membrane processing. However, coiling phagocytosis may show different features with different microbes.
此前，螺旋吞噬作用仅偶然被观察到，且尚未对这种摄取机制进行系统研究。因此，开展了一项比较电子显微镜研究。将不同的人类和小鼠细胞群体、来自各种脊椎动物和无脊椎动物物种的吞噬细胞以及捕食性变形虫与伯氏疏螺旋体（已知可诱导螺旋吞噬作用的微生物之一）一起孵育，以研究所使用的摄取机制。在该模型中，在脊椎动物和无脊椎动物物种中均观察到螺旋吞噬作用，但在变形虫中未观察到。对于人类和小鼠的细胞，这种摄取机制仅限于髓系来源的吞噬细胞。螺旋状的膜间隙并未形成吞噬体；相反，膜融合之后是膜消散。因此，伯氏疏螺旋体的螺旋化显然是后生动物吞噬细胞使用的一种替代性摄取机制，涉及特殊的膜处理。然而，螺旋吞噬作用可能因不同的微生物而呈现不同特征。
