Tappenden K A, Thomson A B, Wild G E, McBurney M I
Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Canada.
Gastroenterology. 1997 Mar;112(3):792-802. doi: 10.1053/gast.1997.v112.pm9041241.
BACKGROUND & AIMS: Intestinal adaptation is a complex physiological process that is not completely understood. Total parenteral nutrition (TPN) induces intestinal atrophy that is prevented by the systemic administration of short-chain fatty acids (SCFAs) as measured by morphological indices (i.e., mucosal weight and mucosal DNA, RNA, and protein concentration). The aim of this study was to examine the effect of SCFA-supplemented TPN on functional markers of intestinal adaptation.
Forty-eight male Sprague-Dawley rats underwent an 80% jejunoileal resection and jugular catheterization. Rats received standard TPN or an isoenergetic, isonitrogenous TPN supplemented with SCFA (TPN + SCFA). Animals were further randomized to receive nutrient solutions for 3 or 7 days.
Ileal uptakes of D-glucose were higher (P < 0.05) in both TPN + SCFA groups. Expression of glucose transporter (GLUT)2 messenger RNA (mRNA) was higher (P < 0.007) in the TPN + SCFA group at day 3. Expression of sodium-dependent glucose transporter 1 tended to be higher in both TPN + SCFA groups (P = 0.1). Na+,K+-adenosine triphosphatase mRNA was significantly more abundant in the TPN groups. GLUT5 and sucrase-isomaltase mRNA abundance did not differ between groups.
Intravenous SCFAs facilitate intestinal adaptation after resection by increasing basolateral intestinal nutrient transport. The addition of SCFAs to current TPN formulations may be warranted to improve functional characteristics of the gastrointestinal tract.
肠道适应性是一个尚未完全被理解的复杂生理过程。全肠外营养（TPN）会导致肠道萎缩，而通过形态学指标（即黏膜重量以及黏膜DNA、RNA和蛋白质浓度）测量发现，全身给予短链脂肪酸（SCFAs）可预防这种萎缩。本研究的目的是检验补充SCFA的TPN对肠道适应性功能标志物的影响。
48只雄性Sprague-Dawley大鼠接受80%空肠回肠切除术并进行颈静脉插管。大鼠接受标准TPN或补充了SCFA的等能量、等氮TPN（TPN + SCFA）。动物被进一步随机分组，接受营养液3天或7天。
两个TPN + SCFA组的回肠D-葡萄糖摄取均更高（P < 0.05）。在第3天，TPN + SCFA组的葡萄糖转运蛋白（GLUT）2信使核糖核酸（mRNA）表达更高（P < 0.007）。两个TPN + SCFA组中钠依赖性葡萄糖转运蛋白1的表达均有升高趋势（P = 0.1）。TPN组中Na⁺,K⁺-三磷酸腺苷酶mRNA明显更为丰富。各组间GLUT5和蔗糖酶-异麦芽糖酶mRNA丰度无差异。
静脉注射SCFAs通过增加肠道基底外侧营养物质转运促进切除术后的肠道适应性。在当前的TPN配方中添加SCFAs可能有助于改善胃肠道的功能特性。
