Testoni M I, Bolzán A D, Bianchi M S, Bianchi N O
Instituto Multidisciplinario de Biología Celular (IMBICE), La Plata, Argentina.
Mutat Res. 1997 Feb 3;373(2):201-6. doi: 10.1016/s0027-5107(96)00198-4.
The effect of several free radicals scavengers on DNA damage and clastogenesis induced by streptonigrin (SN) in CHO cells was investigated. The addition of the antioxidant enzymes superoxide dismutase and/or catalase on CHO cell cultures did not prevent the induction of DNA and chromosome damage by SN. In fact, when superoxide dismutase was added to the culture medium an increase on the frequency of SN-induced chromosome aberrations was observed. Moreover, the addition of the hydroxyl radicals scavenger mannitol caused a significant increase in DNA and chromosome damage induced by SN. On the contrary, when all the antioxidants mentioned above were added-alone or in different combinations-encapsulated into liposomes, a significant decrease in the yield of SN-induced chromosome aberrations and DNA damage was observed. These findings indicate that free radicals are involved in the production of DNA and chromosome damage by SN and that this damage can be partially inhibited through the incorporation of antioxidants by the cells.
研究了几种自由基清除剂对链黑菌素（SN）诱导的中国仓鼠卵巢（CHO）细胞DNA损伤和染色体断裂的影响。在CHO细胞培养物中添加抗氧化酶超氧化物歧化酶和/或过氧化氢酶并不能阻止SN诱导的DNA和染色体损伤。事实上，当向培养基中添加超氧化物歧化酶时，观察到SN诱导的染色体畸变频率增加。此外，添加羟基自由基清除剂甘露醇会导致SN诱导的DNA和染色体损伤显著增加。相反，当上述所有抗氧化剂单独或以不同组合封装在脂质体中添加时，观察到SN诱导的染色体畸变和DNA损伤产量显著降低。这些发现表明，自由基参与了SN诱导的DNA和染色体损伤的产生，并且这种损伤可以通过细胞摄取抗氧化剂而得到部分抑制。
