Bolzán A D, Bianchi N O, Bianchi M S
Instituto Multidisciplinario de Biología Celular (IMBICE), C.C. 403, 1900, La Plata, Argentina.
Mutat Res. 1998 Sep 25;418(1):35-42. doi: 10.1016/s1383-5718(98)00107-7.
The effects of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and the hydroxyl radical scavenger mannitol (MAN) on the clastogenesis induced by STZ in Chinese hamster ovary (CHO) and mosquito cells were investigated. The addition of liposome-entrapped SOD, CAT and MAN to both cell lines caused a significant decrease in the yield of STZ-induced chromosome aberrations (p<0.05). However, the inhibitory effect was more evident in CHO (40.6-67.5%) than in mosquito (15.2-53.6%) cells. These findings indicate that the chromosome damage induced by STZ can be partially inhibited through the incorporation of antioxidant compounds into the cells and suggest that free radicals are involved in the clastogenesis by STZ.
研究了抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）以及羟自由基清除剂甘露醇（MAN）对链脲佐菌素（STZ）在中国仓鼠卵巢（CHO）细胞和蚊子细胞中诱导的染色体断裂作用的影响。将脂质体包裹的SOD、CAT和MAN添加到这两种细胞系中，均导致STZ诱导的染色体畸变率显著降低（p<0.05）。然而，抑制作用在CHO细胞（40.6 - 67.5%）中比在蚊子细胞（15.2 - 53.6%）中更为明显。这些发现表明，通过将抗氧化化合物导入细胞，可以部分抑制STZ诱导的染色体损伤，并提示自由基参与了STZ诱导的染色体断裂作用。
