Enderlin F E, Honohan T
Lab Anim Sci. 1977 Aug;27(4):490-3.
Bile was collected from the rat continuously, over an extended period, using an externalized cannula brought through an intrascapular incision. The cannula was protected by a 30-cm, 16-gauge stainless steel tube attached to the skin through the incision. The rat was housed in a 10.2 x 10.2 x 12.7 cm cage at a lever higher than the collection vial to aid in drainage of bile. A hole in the vial cap permitted the cannula to turn freely as the rat moved about the cage. This method allowed bile to be collected conveniently over long periods of time with minimal restraint of the animal.
通过肩胛间切口引出的外置插管，在较长时间内连续收集大鼠胆汁。插管由一根30厘米长、16号的不锈钢管保护，该不锈钢管通过切口与皮肤相连。大鼠饲养在一个10.2×10.2×12.7厘米的笼子里，放置在高于收集瓶的位置，以利于胆汁引流。收集瓶盖上的一个孔允许插管在大鼠在笼中活动时自由转动。这种方法能够在对动物限制最小的情况下，方便地长时间收集胆汁。
