Heitmeyer S A, Powers J F
Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239-8707.
Lab Anim Sci. 1992 Jun;42(3):312-5.
We describe an improved method for continuous collection of bile from unrestrained rats. Use of an externally accessible, continuous-loop cannula when cannulating the common bile duct allows for full recovery from anesthetic effects and maintenance of a normal bile salt pool until the cannula loop is cut. Bile resulting from the cut cannula is diverted into a surgically implanted glass collection vessel and removed periodically via an externalized sampling port. Bile flow over a 24-hour collection period averaged 0.98 +/- 0.04 ml/hr (Mean +/- SEM, n = 9) with no gross pathological changes noted upon necropsy. This technique offers the capability of reestablishing conditions as close to physiologic as possible postsurgery to minimize potential artifacts during bile collection.
我们描述了一种改进的方法，用于从不受限制的大鼠连续收集胆汁。在插管胆总管时使用外部可触及的连续环套管，可使大鼠从麻醉作用中完全恢复，并维持正常的胆汁盐池，直至切断套管环。切断套管后产生的胆汁被引流到手术植入的玻璃收集容器中，并通过外置采样端口定期取出。在24小时收集期内，胆汁流量平均为0.98±0.04毫升/小时（平均值±标准误，n = 9），尸检时未发现明显的病理变化。该技术能够在手术后尽可能重建接近生理的条件，以尽量减少胆汁收集过程中的潜在假象。
