Chipman J K, Cropper N C
Res Vet Sci. 1977 May;22(3):366-70.
A technique is described for inserting into the bile duct, a re-entrant cannula with a sampling arm to allow intermittent collections of bile from unanaesthetised, minimally restrained rats, for a period of 30 days or more. The flow rate of bile (5.38+/-0-25 cm3/h/kg) was higher than that previously reported when using simple or re-entrant cannulas. This higher flow rate may be a result of allowing a minimum of 10 days post-operative recovery period before starting collection of bile and of avoiding anaesthesia and other stresses at the time of collection.
本文描述了一种技术，即将带有取样臂的折返式插管插入胆管，以便在未麻醉、轻微受限的大鼠身上间歇性采集胆汁，持续30天或更长时间。胆汁流速（5.38±0.25立方厘米/小时/千克）高于此前使用简单插管或折返式插管时所报道的流速。这种较高的流速可能是由于在开始采集胆汁前给予至少10天的术后恢复期，以及在采集时避免麻醉和其他应激因素所致。
