Koumantakis E, Liu W Q, Makrigiannakis A, Relakis C, Unsöld E, Papazoglou T G
University of Crete, Medical School, Department of Gynecology, Heraklion, Greece.
J Photochem Photobiol B. 1997 Jan;37(1-2):96-100. doi: 10.1016/s1011-1344(96)07346-0.
A laser-induced fluorescence-guided biopsy system has been developed for the screening and early detection of malignancies in the female inner/outer genital tract. Fluorescence spectra were recorded during exposure of normal and malignant tissue to He-Cd laser (442 nm) radiation. A characteristic increase in the fluorescence signal at 600 nm for malignant tissue was observed after treatment of the samples with hypocrellin (HA). This, combined with the spectral distribution of tissue natural fluorescence, allowed for the development of simple algorithms, based on the intensity difference. A subsequent index of discrimination between normal and various malignant tissues has been calculated. These results suggest that monitoring of this index during colposcopy could enhance selective detection of the malignant tissue, reducing the risk of leaving pathologic tissue untreated during standard exploratory surgical procedures.
一种激光诱导荧光引导活检系统已被开发出来，用于女性内外生殖道恶性肿瘤的筛查和早期检测。在正常组织和恶性组织暴露于氦镉激光（442nm）辐射期间记录荧光光谱。在用竹红菌素（HA）处理样品后，观察到恶性组织在600nm处的荧光信号有特征性增加。这与组织自然荧光的光谱分布相结合，使得基于强度差异开发简单算法成为可能。随后计算了正常组织与各种恶性组织之间的鉴别指数。这些结果表明，在阴道镜检查期间监测该指数可提高恶性组织的选择性检测，降低在标准探索性手术过程中未治疗病理组织的风险。
