Fallick M L, Sable D B, Faller G, Larock D, Sant G R, Schlesinger R M, Schwaitzberg S D
Department of Urology, New England Medical Center, Tufts University School of Medicine, Boston, MA, USA.
Br J Urol. 1997 Jan;79(1):70-3. doi: 10.1046/j.1464-410x.1997.28015.x.
To evaluate changes in histology and semen variables after using an elastomeric-hydrogel matrix plug as a reversible vasectomy device in dogs, and to determine the potential applications for improving fertility after vasectomy reversal.
Semen samples from six adult male dogs were obtained after left vasal ligation, placement of the plug in the right vas deferens, and subsequent removal of the plug. The vasa, epididymides and testes were evaluated histologically in four of the dogs after removal of the implant.
No sperm were present in the ejaculate after the plug was removed. Histological sections showed intraluminal spermatids in areas where the plug had been placed. The lumen was occluded, secondary to foreign-body giant-cell reaction, at the vasotomy suture site, but other areas of the was previously containing the implant remained patent after it was removed.
There were no sperm in the ejaculate after the plug was removed because the vasal lumen was occluded as a result of an inflammatory reaction to the suture material. In contrast, areas that had contained the implant but without a vasotomy suture were not inflamed. We suggest that further trials comparing various suture materials are carried out to determine if patency rates can be improved during vasotomy closure or vasovasotomy.
评估在犬类中使用弹性水凝胶基质栓作为可逆性输精管结扎装置后组织学和精液变量的变化，并确定输精管复通术后改善生育能力的潜在应用。
在左侧输精管结扎、将栓子置入右侧输精管并随后取出栓子后，采集6只成年雄性犬的精液样本。在取出植入物后，对其中4只犬的输精管、附睾和睾丸进行组织学评估。
取出栓子后，射精中无精子。组织学切片显示，在放置栓子的区域内有管腔内精子细胞。由于异物巨细胞反应，输精管切开缝合部位的管腔被阻塞，但先前放置植入物的输精管其他区域在取出后仍保持通畅。
取出栓子后射精中无精子，这是因为对缝合材料的炎症反应导致输精管腔阻塞。相比之下，含有植入物但没有输精管切开缝合的区域没有炎症。我们建议进行进一步试验比较各种缝合材料，以确定在输精管切开闭合或输精管吻合术中是否可以提高通畅率。
