Stefanski V, Solomon G F, Kling A S, Thomas J, Plaeger S
Norman Cousins Program in Psychoneuroimmunology, UCLA, Los Angeles, California, 90095, USA.
Brain Behav Immun. 1996 Dec;10(4):364-79. doi: 10.1006/brbi.1996.0032.
The impact of social defeat on lymphocyte subpopulations and T helper subsets was investigated in Long Evans rats. CD4 T helper cell subsets with distinct functional properties and different cytokine profiles can be distinguished by using the mAbs OX-22 (anti-CD45RC) and OX-7 (anti-CD90, Thy1.1). Male intruders were exposed for 2, 6, or 48 h to aggressive resident pairs. All intruders were attacked upon introduction and were defeated as indicated by frequent display of full submissive postures. After 2 and 48 h of confrontation, drastic but differential effects on blood leukocyte numbers, CD4 and CD8a cells, and CD4 subsets were evident. However, after 6 h of confrontation most lymphocyte subset numbers corresponded to baseline levels. Focusing on CD4 subsets after 2 h of confrontation, we demonstrated that only the number of the CD45RC-CD90(-) subset declines, whereas neither the number of the CD45RC+CD90(-) subset nor the number of the CD45RC-CD90(+) subset (recent thymic emigrants) was influenced. Con A stimulation of sorted subsets identified the CD45RC-CD90(-) as a poor producer of IFN-gamma. The data clearly demonstrate that social factors might differentially influence not only T cell subsets but also T helper cell subsets with distinct cytokine profiles in a possibly time-dependent manner. Such a stress-induced shift toward a CD45RC+CD90(-)-dominated milieu may have important consequences in interpreting results obtained from mitogenic stimulation of blood lymphocytes and cytokine production profiles measured after such a stimulation. In addition, a shift toward a CD45RC+CD90(-) dominance may modify the type and magnitude of immune response, at least temporarily.
在Long Evans大鼠中研究了社会挫败对淋巴细胞亚群和T辅助细胞亚群的影响。通过使用单克隆抗体OX - 22（抗CD45RC）和OX - 7（抗CD90，Thy1.1），可以区分具有不同功能特性和不同细胞因子谱的CD4 T辅助细胞亚群。雄性入侵者被暴露于具有攻击性的常住配对大鼠2、6或48小时。所有入侵者在被引入时均遭到攻击，并因频繁表现出完全顺从姿势而被击败。在对抗2小时和48小时后，对血液白细胞数量、CD4和CD8a细胞以及CD4亚群产生了显著但不同的影响。然而，在对抗6小时后，大多数淋巴细胞亚群数量与基线水平相对应。在对抗2小时后聚焦于CD4亚群，我们发现只有CD45RC - CD90(-)亚群的数量下降，而CD45RC + CD90(-)亚群和CD45RC - CD90(+)亚群（近期胸腺迁出细胞）的数量均未受影响。对分选后的亚群进行刀豆蛋白A刺激，确定CD45RC - CD90(-)是干扰素 - γ的低产者。数据清楚地表明，社会因素可能以时间依赖性方式不仅对T细胞亚群而且对具有不同细胞因子谱的T辅助细胞亚群产生不同影响。这种应激诱导的向以CD45RC + CD90(-)为主的环境转变可能对解释从血液淋巴细胞的丝裂原刺激获得的结果以及这种刺激后测量的细胞因子产生谱具有重要意义。此外，向CD45RC + CD90(-)优势的转变可能至少暂时改变免疫反应的类型和强度。
