Lee H L, Gregorio E R, Khadri M S, Seleena P
Division of Medical Entomology, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
J Am Mosq Control Assoc. 1996 Dec;12(4):651-5.
Evaluation of the effectiveness of Bacillus thuringiensis ssp. israelensis (B.t.i.) against mosquito larvae dispersed by ultralow volume (ULV) spraying was conducted in simulated field trials. Effectiveness was measured using 3 different indicators: larval mortality, colony-forming unit enumeration, and droplet analysis. B.t.i. was dispersed with a ULV generator using 2 different flow rates: 0.3 and 0.5 liter/min on 2 different days. Based on the results of this study, it can be concluded that an output of 0.3 liter/min is effective for controlling Aedes aegypti. although a dosage of 0.5 liter/min can be used when high residual activity is desired. For Culex quinquefasciatus control, both dosages were effective but with low residual activity. For Anopheles maculatus control, only a discharge rate of 0.5 liter/min was effective with low residual activity. B.t.i. application at both dosages penetrated tires well, indicating that B.t.i. ULV application is an effective method for controlling container-inhabiting mosquitoes. Good coverage of target area and penetration were attributed to satisfactory droplet profiles.
在模拟田间试验中，对苏云金芽孢杆菌以色列亚种（B.t.i.）采用超低容量（ULV）喷雾法杀灭分散的蚊虫幼虫的效果进行了评估。使用3种不同指标来衡量效果：幼虫死亡率、菌落形成单位计数和液滴分析。使用ULV发生器以2种不同流速（0.3和0.5升/分钟）在2个不同日期分散B.t.i.。基于本研究结果，可以得出结论，0.3升/分钟的输出量对控制埃及伊蚊有效。不过，当需要高残留活性时，可以使用0.5升/分钟的剂量。对于致倦库蚊的控制，两种剂量均有效，但残留活性较低。对于多斑按蚊的控制，只有0.5升/分钟的排放速率有效，且残留活性较低。两种剂量的B.t.i.应用均能很好地渗透到轮胎中，这表明B.t.i.的ULV应用是控制栖息于容器中的蚊虫的有效方法。目标区域的良好覆盖和渗透归因于令人满意的液滴分布。
