Seleena P, Lee H L, Nazni W A, Rohani A, Kadri M S
Division of Medical Entomology, Institute for Medical research, Jalan Pahang, Kuala Lumpur, Malaysia.
Southeast Asian J Trop Med Public Health. 1996 Sep;27(3):628-32.
In an effort to develop a more effective technique in dispersing a microbial control agent, Bacillus thuringiensis (Bt), a truck-mounted ultra low volume (ULV) generator (Scorpion) was used to disperse B. thuringiensis israelensis (Bti) and Bti with malathion. Complete larval and adult mortalities for all tested mosquito species within the first 70-80 feet from the ULV generator were achieved. Beyond that distance less than 50% mortality was achieved as insufficient sprayed particles reached the area. A minimum of 10(3) Bti colony forming units per ml is required to cause 100% larval mortality. The sprayed Bti larvicidal toxins were persistent in the test water 7 days post ULV. The effectiveness of B. thuringiensis jegathesan (Btj), a new mosquitocidal Bt serotype was also evaluated. Similar mortality results as Bti were achieved except that the Btj toxins underwent degradation in the test water, since less than 50% less in larval mortality was observed in 7 days post ULV samples. This ULV method has the potential to disperse Bt and malathion effectively for a simultaneous control of mosquito adults and larvae.
为了开发一种更有效的分散微生物控制剂苏云金芽孢杆菌（Bt）的技术，使用了一种车载超低容量（ULV）发生器（蝎子型）来分散以色列亚种苏云金芽孢杆菌（Bti）以及Bti与马拉硫磷的混合物。在距ULV发生器前70 - 80英尺范围内，所有受试蚊虫种类的幼虫和成虫均实现了完全死亡。超过该距离后，由于到达该区域的喷雾颗粒不足，死亡率低于50%。每毫升至少需要10³个Bti菌落形成单位才能导致100%的幼虫死亡率。ULV处理7天后，喷雾的Bti杀幼虫毒素在测试水中仍然存在。还评估了一种新的杀蚊Bt血清型——杰氏亚种苏云金芽孢杆菌（Btj）的效果。除了Btj毒素在测试水中会降解外，其死亡率结果与Bti相似，因为在ULV处理7天后的样本中观察到幼虫死亡率降低了不到50%。这种ULV方法有潜力有效分散Bt和马拉硫磷，同时控制蚊虫成虫和幼虫。
