Randone B, Sterpetti A V, Stipa F, Proietti P, Aromatario C, Guglielmi M B, Palestini M, Santoro-D'Angelo L, Cavallaro A, Cucina A
Department of Surgery, University of Rome, La Sapienza, Italy.
Eur J Vasc Endovasc Surg. 1997 Jan;13(1):66-71. doi: 10.1016/s1078-5884(97)80053-6.
To analyse the role of growth factors (platelet derived growth factor, PDGF; basic fibroblast growth factor, bFGF; interleukin 1, IL-1) in the genesis of myointimal hyperplasia in arterial allografts.
Two groups of experiments were performed: isografts and allografts. The isograft group consisted of 15 inbred Lewis rats in which a 1 cm long segment of aorta was inserted as an abdominal aortic interposition graft. The aortic segments were obtained from syngenic Lewis rats. The allograft group consisted of 15 inbred Lewis rats, in which a 1 cm long segment of aorta was interposed at the abdominal aorta level. The aortic segments were obtained from allogenic Brown-Norway rats.
The animals were killed 4 weeks after surgery and were analysed by morphometric analysis (n = 3 for each group). In addition, production of PDGF, bFGF and IL-1 by aortic segments (n = 12 for each group) in organ culture was assessed.
Allografts had more myointimal hyperplasia, than isografts (p < 0.05). PDGF and bFGF production, generally considered to be the cause of myointimal hyperplasia, was not increased in allografts. IL-1 production was higher in allografts (p < 0.001).
Myointimal hyperplasia in aortic allografts is dependent on growth factors produced by the graft itself. These growth factors are different from PDGF and bFGF that generally have been implicated in the genesis of naturally occurring myointimal hyperplasia and atherosclerosis. IL-1 may have a principal role in the genesis of myointimal hyperplasia in arterial allografts.
分析生长因子（血小板衍生生长因子，PDGF；碱性成纤维细胞生长因子，bFGF；白细胞介素1，IL-1）在同种异体动脉移植肌内膜增生形成中的作用。
进行了两组实验：同基因移植和同种异体移植。同基因移植组由15只近交系Lewis大鼠组成，将一段1厘米长的主动脉作为腹主动脉间置移植物植入。主动脉段取自同基因Lewis大鼠。同种异体移植组由15只近交系Lewis大鼠组成，将一段1厘米长的主动脉在腹主动脉水平进行间置移植。主动脉段取自异体Brown-Norway大鼠。
术后4周处死动物，并通过形态计量分析进行分析（每组n = 3）。此外，评估器官培养中主动脉段（每组n = 12）产生的PDGF、bFGF和IL-1。
同种异体移植的肌内膜增生比同基因移植更多（p < 0.05）。通常被认为是肌内膜增生原因的PDGF和bFGF产生量在同种异体移植中并未增加。同种异体移植中IL-1的产生量更高（p < 0.001）。
同种异体主动脉移植中的肌内膜增生依赖于移植物自身产生的生长因子。这些生长因子不同于通常与自然发生的肌内膜增生和动脉粥样硬化形成有关的PDGF和bFGF。IL-1可能在同种异体动脉移植肌内膜增生的形成中起主要作用。
