Ishizaka S, Saito S, Yoshikawa M, Kimoto M, Nishiyama T
Department of Parasitology, Nara Medical University, Kashihara, Japan.
Cytokine. 1996 Nov;8(11):837-43. doi: 10.1006/cyto.1996.0112.
The supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. The supernatants from transforming growth factor beta (TGF-beta)-treated C3H/HeN spleen cells augmented greatly polyclonal antibody responses compared with the untreated supernatants. IL-10 production and expression of mRNA for IL-10 in Clone 1469 cells was detected by using enzyme-linked immunosorbent assay, ELISPOT assay and PCR amplification. TGF-beta led to augmentation of IL-10 secretion from Clone 1469 cells.
正常肝细胞（克隆1469）培养的上清液可增强小鼠脾细胞中多克隆抗体的产生，但对人外周血淋巴细胞无此作用。与未处理的上清液相比，转化生长因子β（TGF-β）处理的C3H/HeN脾细胞的上清液极大地增强了多克隆抗体反应。通过酶联免疫吸附测定、ELISPOT测定和PCR扩增检测克隆1469细胞中IL-10的产生及IL-10 mRNA的表达。TGF-β导致克隆1469细胞中IL-10分泌增加。
