Watanabe Y, Tsurui H, Ueda T, Furusihima-Shimogawara R, Takamiya S, Kita K, Nishikawa K, Watanabe K
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Biochim Biophys Acta. 1997 Feb 7;1350(2):119-22. doi: 10.1016/s0167-4781(96)00211-4.
Mitochondrial tRNA(Arg) from a nematode, Ascaris suum, was purified and sequenced at the RNA level. An unmodified adenosine was found to exist at the anticodon first position, suggesting that, contrary to the conventional wobble rule, the anticodon ACG of the tRNA can translate all the CGN codons.
从线虫猪蛔虫中纯化出线粒体tRNA(精氨酸),并在RNA水平上进行测序。发现在反密码子的第一位存在一个未修饰的腺苷,这表明,与传统的摆动规则相反,该tRNA的反密码子ACG可以翻译所有的CGN密码子。