Burgess D, Penton A, Dunsmuir P, Dooner H
DNA Plant Technology Corporation, Oakland, CA 94608, USA.
Plant Mol Biol. 1997 Feb;33(3):431-44. doi: 10.1023/a:1005752311130.
Three ADP-glucose pyrophosphorylase (ADPG-PPase) cDNA clones have been isolated and characterized from a pea cotyledon cDNA library. Two of these clones (Psagps1 and Psagps2) encode the small subunit of ADPG-PPase. The deduced amino acid sequences for these two clones are 95% identical. Expression of these two genes differs in that the Psagps2 gene shows comparatively higher expression in seeds relative to its expression in other tissues. Psagps2 expression also peaks midway through seed development at a time in which Psagps1 transcripts are still accumulating. The third cDNA isolated (Psagp11) encodes the large subunit of ADPG-PPase. It shows greater selectivity in expression than either of the small subunit clones. It is highly expressed in sink organs (seed, pod, and seed coat) and undetectable in leaves.
已从豌豆子叶cDNA文库中分离并鉴定出三个ADP - 葡萄糖焦磷酸化酶（ADPG - PPase）cDNA克隆。其中两个克隆（Psagps1和Psagps2）编码ADPG - PPase的小亚基。这两个克隆推导的氨基酸序列有95%的同一性。这两个基因的表达有所不同，Psagps2基因在种子中的表达相对于在其他组织中的表达较高。Psagps2的表达在种子发育中期达到峰值，此时Psagps1转录本仍在积累。分离出的第三个cDNA（Psagp11）编码ADPG - PPase的大亚基。它在表达上比任何一个小亚基克隆都具有更高的选择性。它在库器官（种子、豆荚和种皮）中高度表达，而在叶片中检测不到。
