Chervonskaya E A, Belova G I, Maslova S V, Kutateladze T N, Drozdov S G, Mulders M, Pallansch M A, Kew O M, Agol V I
A. N. Belozersky Institute of Physical-Chemical Biology, Moscow State University, Russia.
J Gen Virol. 1995 Jul;76 ( Pt 7):1687-99. doi: 10.1099/0022-1317-76-7-1687.
A 150 nucleotide long region corresponding to adjoining segments of the genes encoding polypeptides VP1 and 2A of 84 poliovirus strains recently isolated from patients with paralytic poliomyelitis over the territory of the former Soviet Union (FSU) were characterized by sequencing and/or PCR amplification using specially designed primers. Eighteen isolates were found to be very closely related to one or another of the three Sabin vaccine strains. Three distinct classes of geographical genotypes (geotypes) were discerned among 42 wild-type (non-Sabin) strains of serotype 1. One such geotype (called A) was widely circulating in 1990-91 in the Caucasian (Azerbaijan and Georgia) as well as Asian (Kyrgyzstan and Turkmenistan) Republics; this geotype exhibited only weak relatedness to known strains isolated outside the FSU. On the other hand, a subset of strains belonging to another geotype (T) of serotype 1, which circulated in 1991 in Tajikistan, demonstrated very close relatedness to contemporaneous strains isolated in Pakistan, India and Jordan. Strains that were somewhat different, but belonging to the same T-geotype, were found also in Moldova and Georgia. Strikingly, the primary structure of the VP1/2A junction of certain T-geotype isolates differed from the corresponding region of Sabin 1 only in 13-15% of positions, thereby not reaching the upper limit accepted for a geotype. This observation raises, though does not prove, the possibility that at least the relevant segment of the T-geotype RNA originated from the vaccine strain. The third geotype of serotype 1 was represented by a single, perhaps imported, isolate. Four distinct subsets of a common geotype (C) were discerned among 24 wild-type isolates belonging to serotype 3. These strains exhibited a broad geographical distribution being found, in particular, in Armenia, Azerbaijan, Georgia, Turkmenistan and Tajikistan; on the other hand, the C-geotype strains exhibited only a relatively distant relatedness to a strain isolated outside of the FSU (in Oman).
利用特别设计的引物，通过测序和/或聚合酶链反应（PCR）扩增，对来自前苏联（FSU）地区近期从麻痹性脊髓灰质炎患者中分离出的84株脊髓灰质炎病毒编码多肽VP1和2A的基因相邻片段对应的150个核苷酸长区域进行了特征分析。发现18株分离株与三种萨宾疫苗株中的一种或另一种密切相关。在42株1型野生型（非萨宾）毒株中识别出三种不同类别的地理基因型（基因型）。其中一种基因型（称为A）在1990 - 1991年在高加索地区（阿塞拜疆和格鲁吉亚）以及亚洲地区（吉尔吉斯斯坦和土库曼斯坦）广泛传播；这种基因型与在前苏联境外分离出的已知毒株仅有微弱的相关性。另一方面，1型另一种基因型（T）的一部分毒株于1991年在塔吉克斯坦传播，与同期在巴基斯坦、印度和约旦分离出的毒株显示出非常密切的相关性。在摩尔多瓦和格鲁吉亚也发现了属于同一T基因型但略有不同的毒株。令人惊讶的是，某些T基因型分离株的VP1/2A连接区的一级结构与萨宾1型相应区域相比，仅有13 - 15%的位置不同，因此未达到基因型所接受的上限。这一观察结果虽然没有证明，但提出了至少T基因型RNA的相关片段源自疫苗株的可能性。1型的第三种基因型仅由一个可能是输入的分离株代表。在属于3型的24株野生型分离株中识别出一种共同基因型（C）的四个不同亚组。这些毒株具有广泛的地理分布，尤其在亚美尼亚、阿塞拜疆、格鲁吉亚、土库曼斯坦和塔吉克斯坦被发现；另一方面，C基因型毒株与在前苏联境外（阿曼）分离出的一株毒株仅有相对较远的相关性。
