Galbraith D N, Nairn C, Clements G B
Regional Virus Laboratory, Ruchill Hospital, Glasgow, UK.
J Gen Virol. 1995 Jul;76 ( Pt 7):1701-7. doi: 10.1099/0022-1317-76-7-1701.
This study used phylogenetic analysis based on a region of the 5' non-translated region (5'NTR) of a variety of enteroviral sequences to compare sequences associated with chronic fatigue syndrome (CFS) and those from enteroviruses causing acute infections. Direct sequencing of PCR products was used to obtain the nucleic acid sequences from CFS patients. The inferred phylogenetic tree identified three groupings, one correlating with the diagnosis of CFS. The analysis identified a close relationship between the chronic fatigue enteroviral sequences, and showed that 19/20 were distinct from previously described enteroviruses. These results suggest there is persistence of enterovirus infection in some CFS patients and indicate the presence of distinct novel enterovirus sequences.
本研究基于多种肠道病毒序列的5'非翻译区（5'NTR）区域进行系统发育分析，以比较与慢性疲劳综合征（CFS）相关的序列和引起急性感染的肠道病毒序列。采用PCR产物直接测序法从CFS患者中获取核酸序列。推断的系统发育树确定了三个分组，其中一个与CFS诊断相关。分析确定了慢性疲劳肠道病毒序列之间的密切关系，并表明20个序列中有19个与先前描述的肠道病毒不同。这些结果表明，在一些CFS患者中存在肠道病毒感染的持续性，并表明存在独特的新型肠道病毒序列。
