van Poelwijk F, Prins M, Goldbach R
Department of Virology, Agricultural University Wageningen, The Netherlands.
J Gen Virol. 1997 Mar;78 ( Pt 3):543-6. doi: 10.1099/0022-1317-78-3-543.
The nucleotide sequence of the large (L) genome segment of impatiens necrotic spot virus (INSV) has been determined, and herewith the complete nucleotide sequence of the whole, tripartite genome of this important tospovirus has been elucidated. The L RNA is 8776 nucleotides long and of negative polarity, containing one large ORF on the viral complementary strand. Comparison of the deduced amino acid sequence of the INSV L RNA primary translation product (330.3 kDa) with those of the L RNAs of other members of the family Bunyaviridae reveals that this protein represents the putative viral RNA-dependent RNA polymerase. A cluster dendrogram of the (putative) RNA polymerases indicates that the genera Tospovirus and Tenuivirus, though both encompassing ambisense plant-infecting viruses, have different affinities to the animal-infecting Bunyaviridae, tospoviruses being most closely related to the genus Bunyavirus, and tenuiviruses to the genus Phlebovirus.
凤仙花坏死斑病毒（INSV）大（L）基因组片段的核苷酸序列已被确定，至此，这种重要的番茄斑萎病毒属病毒的整个三分体基因组的完整核苷酸序列已被阐明。L RNA长8776个核苷酸，为负链，在病毒互补链上含有一个大的开放阅读框。将INSV L RNA初级翻译产物（330.3 kDa）的推导氨基酸序列与布尼亚病毒科其他成员的L RNA的氨基酸序列进行比较，结果表明该蛋白代表推定的病毒RNA依赖性RNA聚合酶。（推定的）RNA聚合酶的聚类树状图表明，番茄斑萎病毒属和纤细病毒属虽然都包含双义的植物感染病毒，但它们与感染动物的布尼亚病毒科有不同的亲缘关系，番茄斑萎病毒属与布尼亚病毒属关系最为密切，而纤细病毒属与白蛉病毒属关系密切。
