Bernards A T, de Beaufort A J, Dijkshoorn L, van Boven C P
Department of Medical Microbiology, Leiden University Hospital, The Netherlands.
J Hosp Infect. 1997 Feb;35(2):129-40. doi: 10.1016/s0195-6701(97)90101-8.
Septicaemia caused by Acinetobacter occurred in six infants in the neonatal unit. A total of 18 acinetobacters were isolated from blood cultures, cultures of intravascular catheters, and surveillance cultures. Twelve isolates from the six affected infants were identified as Acinetobacter junii by the use of a novel method, amplified ribosomal DNA restriction analysis (ARDRA). Typing of the organisms using the biochemical profiles of the API 20NE system, antibiogram typing, cell envelope protein electrophoresis, and PCR fingerprinting with two primer sets, ERIC1/ERIC2 and ERIC2/ 1026, showed that these 12 isolates were indistinguishable, whereas the remaining six isolates were different. The six infants recovered after therapy with ciprofloxacin alone in five cases and with a combination of ciprofloxacin and gentamicin in one case. This study showed that A. junii is capable of causing a serious, though non-fatal infection in neonates. The combined use of genotypic and phenotypic methods allowed the rapid separation of epidemic from non-epidemic isolates. It is concluded that for a better understanding of the role of the various Acinetobacter genomic species in human pathology, identification of acinetobacters according to the recent taxonomy is imperative.
新生儿病房中有6名婴儿发生了由不动杆菌引起的败血症。从血培养、血管内导管培养物和监测培养物中总共分离出18株不动杆菌。使用一种新方法——扩增核糖体DNA限制性分析（ARDRA），从6名受感染婴儿中分离出的12株菌株被鉴定为琼氏不动杆菌。利用API 20NE系统的生化图谱、抗菌谱分型、细胞包膜蛋白电泳以及使用两组引物ERIC1/ERIC2和ERIC2/1026进行的PCR指纹分析对这些菌株进行分型，结果显示这12株菌株无法区分，而其余6株菌株则不同。6名婴儿中，5例仅用环丙沙星治疗后康复，1例用环丙沙星和庆大霉素联合治疗后康复。本研究表明，琼氏不动杆菌能够在新生儿中引起严重但非致命的感染。基因型和表型方法的联合使用能够快速区分流行菌株和非流行菌株。得出的结论是，为了更好地了解各种不动杆菌基因组种在人类病理学中的作用，根据最新分类法对不动杆菌进行鉴定势在必行。
