Kerckaert I, De Craemer D, Van Limbergen G
Department of Anatomy, University of Gent, Belgium.
J Inherit Metab Dis. 1995;18 Suppl 1:172-80. doi: 10.1007/BF00711438.
Morphometry of peroxisomes is performed on electron micrographs of ultrathin sections after staining for catalase activity with diaminobenzidine; specific peroxisomal labelling is preferred to guarantee recognition. Peroxisomal number, size, axial ratio and volume parameters are determined and compared to control values. Results from 19 patients with loss of peroxisomal functions are listed. In many patients alterations in peroxisomal morphometric features are found. A brief guideline for interpreting morphometric data is included. Diagnostically relevant morphometric alterations are summarized.
在用二氨基联苯胺对过氧化氢酶活性进行染色后，通过超薄切片的电子显微照片对过氧化物酶体进行形态测定；为确保识别，首选特异性过氧化物酶体标记。确定过氧化物酶体的数量、大小、轴比和体积参数，并与对照值进行比较。列出了19例过氧化物酶体功能丧失患者的结果。在许多患者中发现了过氧化物酶体形态特征的改变。其中包括解释形态测量数据的简要指南。总结了与诊断相关的形态测量改变。
