Sidenius P, Jakobsen J
Brain Res. 1979 Sep 14;173(2):315-30. doi: 10.1016/0006-8993(79)90631-0.
In the present study fast axonal transport was examined in streptozotocin rats with 4 weeks duration in diabetes. Tritiated leucine and 14C-labelled glucosamine were injected into the fifth lumbar ganglion and TCA-soluble as well as insoluble activity were measured in segments of the sciatic nerve at various time intervals. (1) Time from injection until start of fast axonal transport was prolonged in diabetic rats whereas anterograde transport velocity was unchanged. (2) Incorporation of labelled leucine was reduced by 40%, whereas labelled glucosamine incorporation was unchanged. (3) Alterations observed in accumulations of labelled glycoconjugates proximal and distal to a collection crush might represent a decreased amount of retrograde transported material. The changes found in protein and glycoconjugate synthesis and transport could be related to the early reduction in axon calibre and conduction velocity in peripheral nerve of streptozotocin-diabetic rat.
在本研究中，对患有4周糖尿病病程的链脲佐菌素诱导的大鼠的快速轴突运输进行了检测。将氚标记的亮氨酸和14C标记的葡糖胺注入第五腰神经节，并在不同时间间隔测量坐骨神经节段中三氯乙酸可溶性及不可溶性活性。（1）糖尿病大鼠从注射到开始快速轴突运输的时间延长，而顺行运输速度未改变。（2）标记亮氨酸的掺入减少了40%，而标记葡糖胺的掺入未改变。（3）在收集挤压近端和远端标记糖缀合物积累中观察到的改变可能代表逆行运输物质的量减少。在蛋白质和糖缀合物合成及运输中发现的变化可能与链脲佐菌素诱导的糖尿病大鼠外周神经轴突直径和传导速度的早期降低有关。
