酿酒酵母HOC1是pkc1的一个抑制因子，编码一种假定的糖基转移酶。
Saccharomyces cerevisiae HOC1, a suppressor of pkc1, encodes a putative glycosyltransferase.
作者信息
Neiman A M, Mhaiskar V, Manus V, Galibert F, Dean N
机构信息
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215, USA.
出版信息
Genetics. 1997 Mar;145(3):637-45. doi: 10.1093/genetics/145.3.637.
Abstract
The Saccharomyces cerevisiae gene PKC1 encodes a protein kinase C isozyme that regulates cell wall synthesis. Here we describe the characterization of HOC1, a gene identified by its ability to suppress the cell lysis phenotype of pkc1-371 cells. The HOC1 gene (Homologous to OCH1) is predicted to encode a type II integral membrane protein that strongly resembles Och 1p, an alpha-1,6-mannosyltransferase. Immunofluorescence studies localized Hoc1p to the Golgi apparatus. While overexpression of HOC1 rescued the pkc1-371 temperature-sensitive cell lysis phenotype, disruption of HOC1 lowered the restrictive temperature of the pkc1-371 allele. Disruption of HOC1 also resulted in hypersensitivity to Calcofluor White and hygromycin B, phenotypes characteristic of defects in cell wall integrity and protein glycosylation, respectively. The function of HOC1 appears to be distinct from that of OCH1. Taken together, these results suggest that HOC1 encodes a Golgi-localized putative mannosyltransferase required for the proper construction of the cell wall.
摘要
酿酒酵母基因PKC1编码一种调节细胞壁合成的蛋白激酶C同工酶。在此，我们描述了HOC1的特征，该基因是通过其抑制pkc1 - 371细胞的细胞裂解表型的能力而被鉴定出来的。HOC1基因（与OCH1同源）预计编码一种II型整合膜蛋白，它与Och 1p（一种α-1,6-甘露糖基转移酶）非常相似。免疫荧光研究将Hoc1p定位到高尔基体。虽然HOC1的过表达挽救了pkc1 - 371温度敏感型细胞裂解表型，但HOC1的破坏降低了pkc1 - 371等位基因的限制温度。HOC1的破坏还导致对荧光增白剂和潮霉素B过敏，这分别是细胞壁完整性缺陷和蛋白质糖基化缺陷的特征性表型。HOC1的功能似乎与OCH1不同。综上所述，这些结果表明HOC1编码一种高尔基体定位的假定甘露糖基转移酶，它是细胞壁正常构建所必需的。
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