Lagendijk J, Ubbink J B, Delport R, Vermaak W J, Human J A
Department of Chemical Pathology, University of Pretoria, South Africa.
Res Commun Mol Pathol Pharmacol. 1997 Jan;95(1):11-20.
The purpose of the study was to investigate changes in serum ubiquinol/ubiquinone ratio with copper ion induced oxidative stress, and to assess the ubiquinol/ubiquinone ratio as marker of in vivo oxidative stress in patients with coronary artery disease (CAD). Plasma ubiquinol, ubiquinone, vitamin E (alpha-tocopherol) and total cholesterol (TC) concentrations were measured in 40 patients with angiographically confirmed coronary artery disease and 100 apparently healthy controls. The mean (SD) ubiquinol/ubiquinone ratio of 26.5 (7.5) of the CAD patients was significantly lower than the mean ratio of 30.2 (8.8) of the controls (p = 0.02). Our results indicate that the ubiquinol/ubiquinone ratio is a sensitive marker of oxidative stress and that an altered ubiquinol/ubiquinone ratio is the first sign of lipoprotein exposure to oxidative stress. The altered ratio in CAD patients cannot be explained by differences in plasma vitamin E levels. The vitamin E concentrations were in fact significantly higher in CAD patients, and did not appear to protect the ubiquinol from increased oxidation due to free radical reactions. These results may indicate that circulating lipoproteins of CAD patients are more exposed to, or are more susceptible to, free radical reactions compared with apparently healthy controls.
本研究的目的是调查铜离子诱导的氧化应激下血清泛醇/泛醌比值的变化，并评估泛醇/泛醌比值作为冠状动脉疾病（CAD）患者体内氧化应激标志物的情况。对40例经血管造影证实患有冠状动脉疾病的患者和100名明显健康的对照者测量了血浆泛醇、泛醌、维生素E（α-生育酚）和总胆固醇（TC）浓度。CAD患者的平均（标准差）泛醇/泛醌比值为26.5（7.5），显著低于对照组的平均比值30.2（8.8）（p = 0.02）。我们的结果表明，泛醇/泛醌比值是氧化应激的敏感标志物，且泛醇/泛醌比值的改变是脂蛋白暴露于氧化应激的首个迹象。CAD患者中该比值的改变不能用血浆维生素E水平的差异来解释。事实上，CAD患者的维生素E浓度显著更高，且似乎并未保护泛醇免受自由基反应导致的氧化增加。这些结果可能表明，与明显健康的对照者相比，CAD患者的循环脂蛋白更易暴露于自由基反应，或对自由基反应更敏感。
