Otter A, Wilson B W, Scholes S F, Jeffrey M, Helmick B, Trees A J
Veterinary Investigation Centre, Johnstown, Carmarthen.
Vet Rec. 1997 Feb 15;140(7):175-7.
Between January and April 1995, the brains and hearts of 281 aborted lambs derived from 209 submissions to veterinary investigation centres in England and Wales were examined histologically. One-hundred-and-seventy-nine samples of fetal pleural fluid from these lambs and 141 from lambs not examined histologically were examined for antibodies to Neospora by an indirect fluorescent antibody test. Non-suppurative myocarditis and encephalitis were present in nine lambs. Immunocytochemistry using antisera against Neospora caninum and Sarcocystis species resulted in no labelling but anti-sera to Toxoplasma gondii revealed labelled organisms in four lambs. No significant antibody titres against Neospora were detected in any of the samples of fetal pleural fluid. These results suggest that Neospora infection is not associated with significant numbers of abortions in sheep in England and Wales.
1995年1月至4月期间，对来自英格兰和威尔士兽医调查中心的209份送检样本中的281只流产羔羊的大脑和心脏进行了组织学检查。对这些羔羊的179份胎儿胸水样本以及141份未进行组织学检查的羔羊的胸水样本，采用间接荧光抗体试验检测了抗新孢子虫抗体。9只羔羊出现了非化脓性心肌炎和脑炎。使用抗犬新孢子虫和肉孢子虫属抗血清进行免疫细胞化学检测未发现标记，但抗刚地弓形虫抗血清在4只羔羊中显示有标记的生物体。在任何胎儿胸水样本中均未检测到针对新孢子虫的显著抗体滴度。这些结果表明，在英格兰和威尔士，新孢子虫感染与大量绵羊流产无关。
