Otter A, Jeffrey M, Griffiths I B, Dubey J P
Veterinary Investigation Centre, Cambridge.
Vet Rec. 1995 Jun 17;136(24):602-6. doi: 10.1136/vr.136.24.602.
Selected brains and fetal viscera from 190 aborted or stillborn bovine fetuses submitted to Veterinary Investigation Centres in England and Wales between August 1992 and January 1993 were examined histologically. Non-suppurative inflammation of the brain and/or myocardium and placental cotyledons was identified by light microscopy in 20 (10.5 per cent). An immunocytochemical examination of fixed tissue sections using antisera against Neospora caninum, Toxoplasma gondii and Sarcocystis species revealed positive immunolabelling for N caninum in the brains of eight (4.2 per cent), but no labelling with anti-T gondii or anti-Sarcocystis species antisera was evident. These results suggest that N caninum may be an important cause of reproductive failure in cattle in England and Wales.
对1992年8月至1993年1月间提交给英格兰和威尔士兽医调查中心的190例流产或死产牛胎儿的大脑和胎儿内脏进行了组织学检查。通过光学显微镜在20例（10.5%）中发现了大脑和/或心肌以及胎盘小叶的非化脓性炎症。使用抗犬新孢子虫、弓形虫和肉孢子虫属抗血清对固定组织切片进行免疫细胞化学检查，结果显示在8例（4.2%）的大脑中犬新孢子虫免疫标记呈阳性，但未发现抗弓形虫或抗肉孢子虫属抗血清的标记。这些结果表明，犬新孢子虫可能是英格兰和威尔士牛繁殖失败的一个重要原因。
