MacGregor B J, Moser D P, Alm E W, Nealson K H, Stahl D A
Department of Civil Engineering, Northwestern University, Evanston, Illinois 60201, USA.
Appl Environ Microbiol. 1997 Mar;63(3):1178-81. doi: 10.1128/aem.63.3.1178-1181.1997.
RNA from Lake Michigan sediment was hybridized with a DNA probe for archaeal 16S rRNA. There was a peak of archaeal rRNA abundance in the oxic zone and another immediately below it. Six contributing species were identified by PCR amplification of extracted DNA with primers specific for archaeal rDNA: two related to Methanosarcina acetivorans and four related to marine crenarchaeotal sequences. rRNA quantification using a DNA probe specific for this crenarchaeotal assemblage showed it is most abundant in the oxic zone, where it accounts for about 10% of total archaeal rRNA.
从密歇根湖沉积物中提取的RNA与用于古菌16S rRNA的DNA探针进行杂交。在有氧区域存在一个古菌rRNA丰度峰值，且在其正下方还有一个峰值。通过用针对古菌rDNA的特异性引物对提取的DNA进行PCR扩增，鉴定出了六个有贡献的物种：两个与嗜乙酸甲烷八叠球菌相关，四个与海洋泉古菌序列相关。使用针对这种泉古菌组合的特异性DNA探针进行rRNA定量分析表明，它在有氧区域最为丰富，在该区域它约占古菌总rRNA的10%。
