Devereux R, Mundfrom G W
U.S. Environmental Protection Agency, Gulf Breeze, Florida.
Appl Environ Microbiol. 1994 Sep;60(9):3437-9. doi: 10.1128/aem.60.9.3437-3439.1994.
The divergence of 16S rDNA sequences in marine sediment was investigated. Twenty unique partial sequences were found among 33 cloned following PCR. Thirteen shared 82 to 91% similarity with sequences of delta subclass sulfate-reducing bacteria. Three contained the target sequence for a sulfate-reducing bacterium-specific oligonucleotide probe designed from pure-culture studies.
对海洋沉积物中16S rDNA序列的差异进行了研究。PCR扩增后克隆得到的33个序列中发现了20个独特的部分序列。其中13个与δ亚类硫酸盐还原菌的序列具有82%至91%的相似性。3个序列含有根据纯培养研究设计的硫酸盐还原菌特异性寡核苷酸探针的靶序列。