Singh D, Pandey R S
Animal Biochemistry Division, National Dairy Research Institute, Karnal, India.
Indian J Exp Biol. 1996 Nov;34(11):1158-60.
Glutathione-S-transferases (GSTs) are drug metabolizing and detoxification enzymes, involved in the intracellular transport and metabolism of steroid hormones. This study indicated that the enzyme was heterogeneously distributed and changed during estrous cycle. The enzyme was found to be predominantly located in the cytosolic fraction while considerable activity was also observed in the mitochondrial and microsomal fractions. The activity of GST was measured during different stages of estrous cycle, viz proestrous, estrous, metestrous and diestrous. The change in its activity was observed during different stages of estrous cycle. Estrous stage was observed to have the highest GST activity. Immature (28-29 days old) rats treated with estradiol-17 beta (5 micrograms in 0.2 ml oil/animal/day, and 10 micrograms in 0.2 ml oil/animal/day, for 3 days), ovarian GST activity seemed to increase significantly (P < 0.01). This increase in the activity and the heterogeneous distribution of GST indicates the functional role of this enzyme in the ovary under endocrine regulation.
谷胱甘肽 - S - 转移酶（GSTs）是药物代谢和解毒酶，参与类固醇激素的细胞内运输和代谢。本研究表明该酶分布不均一且在发情周期中发生变化。发现该酶主要位于胞质部分，同时在线粒体和微粒体部分也观察到相当的活性。在发情周期的不同阶段，即动情前期、发情期、动情后期和动情间期，测定了GST的活性。在发情周期的不同阶段观察到其活性变化。发情期的GST活性最高。用17β - 雌二醇（5微克/动物/天，溶于0.2毫升油中；10微克/动物/天，溶于0.2毫升油中，处理3天）处理未成熟（28 - 29日龄）大鼠，卵巢GST活性似乎显著增加（P < 0.01）。GST活性的这种增加及其不均一分布表明该酶在内分泌调节下在卵巢中的功能作用。
