College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Toxicol Lett. 2012 Aug 3;212(3):329-36. doi: 10.1016/j.toxlet.2012.06.006. Epub 2012 Jun 19.
The regulation of antioxidant enzymes has received increased attention in terms of protection from many diseases. Despite reports that administered estradiol derivatives can change antioxidant enzyme levels, comprehensive information is not available regarding the effects of the human menstrual cycle or the rat estrous cycle on the expression of the antioxidant enzyme system. The present study was performed to determine the expression levels of cytosolic antioxidant enzymes, including superoxide dismutase-1, catalase, glutathione peroxidase-1, glutathione reductase, peroxiredoxin (Prx)-1, Prx-2, thioredoxin-1, gamma-glutamylcysteine ligase catalytic subunit, alpha-class glutathione S-transferase (GST), pi-class GST, and mu-class GST, in the liver and ovaries of female rats during diestrus and proestrus. Our results indicate that hepatic expression of Prx-1 and Prx-2, and ovarian expression of alpha-class GST were increased significantly during the proestrus phase compared with the diestrus phase. These results suggest that the hepatic Prx family and ovarian alpha-class GST are sensitive to changes during the estrous cycle. Further studies are needed to determine the physiological significance of the regulation of the Prx family and alpha-class GST during the estrous cycle.
抗氧化酶的调节在预防许多疾病方面受到了越来越多的关注。尽管有报道称，施用雌激素衍生物可以改变抗氧化酶水平，但关于人类月经周期或大鼠发情周期对抗氧化酶系统表达的影响的综合信息尚不清楚。本研究旨在确定在发情间期和发情前期，雌性大鼠肝脏和卵巢中细胞质抗氧化酶的表达水平，包括超氧化物歧化酶-1、过氧化氢酶、谷胱甘肽过氧化物酶-1、谷胱甘肽还原酶、过氧化物酶-1、过氧化物酶-2、硫氧还蛋白-1、γ-谷氨酰半胱氨酸连接酶催化亚基、α 类谷胱甘肽 S-转移酶 (GST)、π 类 GST 和 μ 类 GST。我们的结果表明，与发情间期相比，发情前期肝脏中 Prx-1 和 Prx-2 的表达以及卵巢中 α 类 GST 的表达显著增加。这些结果表明，肝脏的 Prx 家族和卵巢的 α 类 GST 对发情周期的变化敏感。需要进一步的研究来确定 Prx 家族和 α 类 GST 在发情周期中的调节的生理意义。
